Replenishing glutathione counters ciprofloxacin-induced acute liver failure via possible gene modifying mechanism

Background: Acute liver failure (ALF) can be defined as rapid loss of functions even in the absence pre-existing disorders. ALF is a medical emergency that necessitates hospitalization and, if not properly treated, transplant may only cure. Herein, we studied potential therapeutic effects exogenous glutathione on drug-induced ALF. Materials: Thirty male Wistar albino rats were used present study. Rats divided (10/group) into: control group (received intra-peritoneal injection distilled water), ciprofloxacin-treated 800 mg/kg/day ciprofloxacin for 15 consequent days with concomitant and + glutathione-treated 200 glutathione). Measurement serum ALT, AST, GGT LDH enzymes activities performed, hepatic gene expression CYP3A, GPx, GSR Nrf2 mRNA was assessed. Results: Treatment resulted significant increase when compared to group. Ciprofloxacin also induced down-regulation corresponding values Glutathione administration significantly normalized up-regulated expression. Conclusion: could play roles treatment acute by preventing oxidative stress-induced disruption hepatocyes cell membranes alteration cytochrome P450 antioxidant genes.